Identification of Major Loci and Candidate Genes for Anthocyanin Biosynthesis in Broccoli Using QTL-Seq

Anthcyanins determine the colors of flowers, fruits, and purple vegetables act as important health-promoting antioxidants. BT 126 represents a broccoli variety with high content anthocyanins (5.72 mg/g FW). Through QTL-seq bulk segregant analysis, present study aimed to quantitative trait loci (QTLs) involved in anthocyanin biosynthesis F2 population (n = 302), which was obtained by crossing non-anthocyanin-containing SN 60. The whole-genome resequencing 30) green segregates detected ~1,117,709 single nucleotide polymorphisms (SNPs) B. oleracea genome. Two QTLs, tightly correlated (p < 0.05), were on chromosomes 7 (BoPur7.1) 9 (BoPur9.1). subsequent high-resolution mapping BoPur9.1 280) F3 580), high-throughput genotyping SNPs technology, narrowed major QTL region physical distance 73 kb, containing 14 genes. Among these genes, Bo9g174880, Bo9g174890, Bo9g174900 showed homology AT5G07990 (gene encoding flavonoid 3? hydroxylase), identified candidate gene for BoPur9.1. expression BoF3’H significantly higher than that SN60. Multiple biomarkers, related represented potential targets marker-assisted selection (MAS) foranthocyanin broccoli. provided genetic insights into development novel crop varieties augmented features improved appearance.